منابع مشابه
Construction of a Synthetic Vector for Preparation of a 100 Base Pair DNA Ladder
DNA size markers are widely used to estimate the size of DNA samples on agarose or polyacrylamide gelelectrophoresis (PAGE). DNA markers can be prepared by mixing PCR products with definite sizes.Alternatively, they are prepared by restriction enzyme digestion of the genomic DNA of bacteriophages ornatural and synthetic DNA plasmids. The present study describes engineering of ...
متن کاملCharacterization of a novel, low-molecular-weight DNA-binding protein from Escherichia coli.
A low-molecular-weight (7000), heat-stable protein--HU--that stimulates transcription of bacteriophage lambda DNA by E. coli RNA polymerase was purified from E. coli extracts using affinity chromatography on DNA-cellulose. HU binds to native DNA, resulting in an apparent thickening of the DNA chains as revealed by electron microscopy. Contrary to DNA unwinding proteins, it causes no destabiliza...
متن کاملStudies on the Preparation of a Novel Supermix Dna Ladder by Restriction Digestion
Modern biological tools of recombinant technology have tremendous potential to characterize DNA molecules with the choice of DNA ladders. Determination of molecular weight of linear and double stranded DNA fragments either on agarose or polyacrylamide gel is done extensively with the DNA ladders. The most popular DNA ladders available in the market range from less than 25bp to 40 kb or more. Am...
متن کاملanti - inflammatory effects of a novel low molecular weight compound
(4939 articles) Immunobiology Articles on similar topics can be found in the following Blood collections http://bloodjournal.hematologylibrary.org/site/misc/rights.xhtml#repub_requests Information about reproducing this article in parts or in its entirety may be found online at: http://bloodjournal.hematologylibrary.org/site/misc/rights.xhtml#reprints Information about ordering reprints may ...
متن کاملLow Molecular Weight Heparin Overdose: A 10 Year Case Series
Background: Low molecular weight heparin (LMWH) is used for the treatment and prevention of coagulative disorders. Few patients receiving therapeutic doses of LMWH develop major hemorrhage. Currently there are few reports in the literature on acute overdose on adults. In this study, clinical profile, treatment and outcome of 21 patients who acutely overdosed enoxaparin are described. Method...
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ژورنال
عنوان ژورنال: Technical Tips Online
سال: 1997
ISSN: 1366-2120
DOI: 10.1016/s1366-2120(08)70043-6